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Eur J Cardiothorac Surg 2004;26:294-300
© 2004 Elsevier Science NL
a Department of Cardiac Surgery, The General Hospital, Southampton, UK
b Department of Cardiac Anaesthesia, The General Hospital, Southampton, UK
c Department of Biomolecular Sciences, University of Portsmouth, Portsmouth, UK
Received 25 December 2003; received in revised form 22 March 2004; accepted 5 April 2004.
* Corresponding author. Address: Department of Cardiac Surgery, Glenfield General Hospital, Groby Rd, Leicester LE3 9QP, UK. Tel.: +44-116-287-1471; fax: +44-116-232-1282
e-mail: alexiou486{at}aol.com
Objective: Leucocyte activation is central to end-organ damage that occurs during cardiac surgery under cardiopulmonary bypass (CPB). Exhaled nitric oxide (NO) increases in inflammatory lung conditions and has been proposed as a marker of pulmonary inflammation during CPB. This study examined the effect of leucodepletion on leucocyte activation, pulmonary inflammation and oxygenation in patients undergoing coronary revascularisation. Methods: Fifty low-risk patients undergoing first time coronary artery bypass graft (CABG) were randomised to two groups. Twenty-five patients had an arterial line leucocyte-depleting filter and 25 controls had a standard filter. Arterial blood samples were taken before CPB, 5 and 30 min on CPB, 5 min after aortic clamp removal and 6 h post-operatively. Activated leucocytes were identified with Nitroblue Tetrazolium staining. NO was sampled via an endotracheal teflon tube 15 min after median sternotomy before CPB and 30 min after discontinuation of CPB using a real-time chemiluminescense analyser. Respiratory index (alveolar-arterial oxygenation index, AaOI) was calculated before CPB, 1, 2, 4, 8 and 18 h post-operatively. Clinical outcome end-points were also recorded. Results: Total and activated leucocyte counts were significantly lower following leucodepletion during CPB (P<0.0001). Exhaled NO rose significantly after CPB in the control group (3.8±1 ppb/s before CPB vs 5.6±2 ppb/s after CPB (P=0.003),) but not in the leucodepleted group (3.7±1 ppb/s before CPB vs 3.9±1 ppb/s after CPB (P=0.51)). AaOIs were consistently lower after leucodepletion (anova, P=0.001). The duration of mechanical ventilation, the intensive care and hospital stay and the frequency of cardiac and respiratory complications were similar in the two groups. Conclusions: Leucodepletion reduces the numbers of circulating activated leucocytes and the pulmonary inflammation during CPB. This appears to limit lung injury and improve oxygenation in low-risk patients undergoing CABG surgery. Larger numbers of patients are required to evaluate the effect of continuous arterial line leucodepletion on the clinical outcome.
Key Words: Coronary artery bypass grafting • Leucocyte activation and depletion • Lung function
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